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Dr. GPCR PodcastAuthor: Dr. Yamina Berchiche
We bring you closer to dedicated scientists who work tirelessly to help understand GPCR pathophysiology. Language: en Genres: Life Sciences, Science Contact email: Get it Feed URL: Get it iTunes ID: Get it Trailer: |
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Imaging GPCRs in Action: Chemical Probes for Next-Level Discovery | Dr. Johannes Broichhagen #178
Episode 178
Tuesday, 2 December, 2025
Chemical probes are reshaping how we map GLP-1R in real time — revealing receptor pools antibodies can’t reliably capture.This is Episode 2 of a 3-part GPCR tool-development series created in partnership with Celtarys Research.Summary:Dr. Johannes Broichhagen aka JB breaks down the design logic behind fluorophore-linked peptides, assay trade-offs, and what true receptor internalization looks like in live tissue. A concise masterclass in assay development and GPCR drug discovery.What you’ll learn:• Why antibody variability pushed JB toward chemical probe engineering• The design logic behind Luxendin-based fluorescent tools — and how structure guides function • What “good assay development” looks like when cells, tissue, and probe behavior collide• Behind-the-scenes stories from the collaboration with David Hodson• Why parallelized experiments matter for reproducibility and signal quality• How small-molecule probes outperform antibodies in live-cell and tissue imaging• The surprising breakthroughs that shifted JB’s entire research trajectory• Future directions: multi-color GPCR mapping, AI-guided ligand design, and in vivo chemical biology Dr GPCR Links & Resources:• Dr. GPCR Ecosystem: https://www.ecosystem.drgpcr.com/ • Membership & Pricing: https://www.ecosystem.drgpcr.com/university-pricing• Weekly News: https://www.ecosystem.drgpcr.com/gpcr-weekly-news
