 |
Listen In - Bitesize Bio Webinar AudiosThe Listen In podcast from Bitesize Bio is a compilation of our best webinars to enjoy at your leisure, wherever and whenever. Author: Bitesize Bio
The Listen In podcast from Bitesize Bio is a compilation of our best webinars to enjoy at your leisure, wherever and whenever. Each episode is an opportunity to gain the valuable insights you need to advance your research. From a crash course in developing fool-proof ELISAs to the latest applications and innovations in CRISPR/Cas9 and microscopy techniques, and much moreyou'll hear about challenges encountered and discover practical solutions to achieve the best possible results. Tap into the experience and expertise of leading researchers and commercial specialists to drive your research projects forward efficiently and productively. Listen In now! https://bitesizebio.com/listen-in/ Language: en Genres: Life Sciences, Science Contact email: Get it Feed URL: Get it iTunes ID: Get it |
Listen Now...
High-Throughput and Wide-Range Protein Concentration Determination of Monoclonal Antibodies
Episode 137
Monday, 24 February, 2025
In this episode of Listen In, explore high-throughput and wide-range protein concentration analysis that can deliver a 20-fold improvement in time and budget savings.Protein concentration is an obligatory critical quality attribute necessary to determine total protein content in biopharmaceutical formulations. Concentration determination is necessary before many downstream assays. A UV-based spectrophotometric assay using the protein’s extinction coefficient is the most typical method. Conventional UV methods or a specialized approach using variable pathlength slope spectroscopy could take one day to measure 15 samples in triplicate. Larger batches would require multiple analysts for a single-day turnaround time. With vast studies spanning multiple projects, protein concentration analysis becomes a key deliverable before all other methods can be performed. Thus, efficient rapid concentration analysis with a minimal volume requirement is essential. Hit play to learn how the Lunatic (Unchained Labs, Pleasanton, CA) offers rapid, plate-based concentration measurement with minimal prep and sample volume requirements. It utilizes only two microliters per sample, can analyze 96 wells in less than 12 minutes, and provides accurate measurements over a broad concentration range, from 0.03 mg/mL to 176 mg/mL.Watch the full presentation here: https://events.bitesizebio.com/high-throughput-and-wide-range/joinBrowse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
