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Absolute Gene-iusAuthor: Jordan Ruggieri, Lisa Crawford
The Applied Biosystems Absolute Gene-ius podcast is a fun and informative dive into the molecular methods behind modern biology. Each episode brings scientists and experts together for lively conversations about the technologies, workflows, discoveries, and career stories shaping life science research. Language: en Genres: Chemistry, Life Sciences, Science Contact email: Get it Feed URL: Get it iTunes ID: Get it Trailer: |
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Where qPCR shines – matching the right PCR technology to the experiment
Episode 19
Wednesday, 4 November, 2026
Newer does not always mean better for the question at hand.In this Science Snapshot episode, Lisa Crawford and Jordan Ruggieri put qPCR back in the spotlight with insights from researchers who use it across a wide range of applications. Dr. John Pfeifer reflects on the evolution of real-time PCR and describes qPCR as a molecular biology “Swiss Army knife.” Dr. Christian Cobaugh explains why his team returned to qPCR as a practical, cost-effective platform for developing and vetting new assays. Dr. Brandi Kiel Reese highlights qPCR’s “sweet spot” for quantifying known genes and transcripts when targets are present at measurable levels, while Lexi Heger shows how targeted and multiplex qPCR assays can distinguish grapevine pathogens and monitor them in environmental samples. Finally, Dr. Guy Novotny explains how qPCR’s broader quantitative range can be an advantage when target abundance is high.All these guests make it clear that it is about matching the PCR technology to the experiment at hand, and that sometimes the best tool is still the classic one. Visit the Absolute Gene-ius page to learn more about the guests, hosts, and science featured in the series. Explore how Applied Biosystems™ QuantStudio™ Digital and Real-Time PCR Systems support research across neuroscience, oncology, agriculture, and more.
